Join DNA Script at ESHG 2022
We invite you to our corporate satellite workshop, poster presentatio, and exhibition booth to learn more about what printing oligos in-house using the SYNTAX System can bring to your labs.
With the SYNTAX System in your lab, you can
- Save Time—Design, print, and test custom primers and probes in a single day
- Reduce Your Waste and Costs—Print just the amount of each probe you need
- Accelerate Your Innovation—Ready-to-use DNA probes lets you finish before others even start
Corporate Satellite Workshop | Sunday, June 12th, 14.15 – 15.45 CEST | Room N1 & 2, Level 1
Join us in a presentation by Jared Nigg, Ph.D., from the Institut Pasteur to learn he used the SYNTAX System to create DNA probes for their smFISH assay to optimize the sensitivity and specificity of the assay. Soheila Beck, Ph.D., from DNA Script, will give a short introduction to the advantages of the SYNTAX System and the power of printing same-day labeled probes and primers for genomics applications.
SYNTAX System – Benchtop Enzymatic Synthesis Delivers On-Demand Fluorescently-Labeled Probes and Same-Day Results
Soheila Beck, Ph.D., Senior Market Development Manager, DNA Script
Single molecule RNA FISH (smFISH) is a powerful tool for the detection of viral RNA in the Drosophila gut
Jared Nigg, Ph.D., Postdoctoral Fellow, Institut Pasteur, Viruses and RNAi unit, Paris, France
Summary: Insect-borne viruses cause millions of infections in humans and no effective programs exist to prevent their transmission. Drosophila melanogaster is a powerful model for elucidating virus-vector interactions, thus enabling development of transmission prevention strategies. Drosophila A virus (DAV) infects the D. melanogaster gut. We investigated the physiology of DAV infection by immunofluorescence to determine which intestinal cell types are infected. Simultaneously, we used single molecule fluorescence in situ hybridization (smFISH) as an alternative to immunofluorescence. SYNTAX System was used to synthesize the oligos which were used to detect DAV or endogenous RNAs. Leveraging the SYNTAX System to print oligos in less than a day, sensitivity and specificity of the smFISH assay could be quickly fine-tuned. Our study demonstrates that smFISH is a rapid and simple alternative to immunofluorescence to study viral tropism.
Poster Presentation (Group D Viewing) | Monday, June 13th, 15:45 – 16:45 | Poster Hall X3
By Enabling Same Day Synthesis of Hydrolysis Probes on the SYNTAX System, Enzymatic DNA Synthesis (EDS) Promises to Unleash the Development of Multiplexed qPCR Assays
Benoit Derrien*, Nadège Tardieu, Henri Lachaize, Anaïs Valette, Erwan Grolleau, Charles, Rouillard, Gabriel De Crozals, Christine Peponnet, Florence Mahé, Xavier Godron
Quantitative PCR (qPCR) was first developed in the early 90’s and has remained one of the gold standard techniques used in modern day gene expression studies. By implementing the synthesis of fluorophore-labeled probes on the SYNTAX System, DNA Script opens access to the rapid on demand synthesis of good quality qPCR probes. In this study, we demonstrate the performance of qPCR probes generated using DNA Script’s proprietary Enzymatic DNA Synthesis (EDS) technology. By combining EDS and labelling chemistry within the SYNTAX System, we enable the fully automated synthesis of 32 qPCR probes and their associated primer pairs in less than 12 hours directly in the lab. By enabling same day synthesis of qPCR probes, the SYNTAX System alleviates one of the bottlenecks associated with qPCR by providing next-day results.